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KMID : 0359320170570010001
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Korean Journal of Veterinary Research
2017 Volume.57 No. 1 p.1 ~ p.7
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Fucoidan attenuates 6-hydroxydopamine-induced neurotoxicity by exerting anti-oxidative and anti-apoptotic actions in SH-SY5Y cells
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Kim Myung-Hwan
Namgoong Hoon
Jung Bae-Dong
Kwon Myung-Sang
Choi Yeon-Shik
Shin Tae-Kyun
Kim Hyoung-Chun
Wie Myung-Bok
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Abstract
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Parkinson’s disease (PD) is an irreversible neurological disorder with related locomotor dysfunction and is characterized by the selective loss of nigral neurons. PD can be experimentally induced by 6-hydroxydopamine (6OHDA). It has been reported that reactive oxygen species, which deplete endogenous glutathione (GSH) levels, may play important roles in the dopaminergic cell death characteristic of PD. Fucoidan, a sulfated algal polysaccharide, exhibits anti-inflammatory and anti-oxidant actions. In this study, we investigated whether fucoidan can protect against 6-OHDA-mediated cytotoxicity in SH-SY5Y cells. Cytotoxicity was evaluated by using MTT and LDH assays. Fucoidan alleviated cell damage evoked by 6-OHDA dose-dependently. Fucoidan reduced the number of apoptotic nuclei and the extent of annexin-V-associated apoptosis, as revealed by DAPI staining and flow cytometry. Elevation of lipid peroxidation and caspase-3/7 activities induced by 6-OHDA was attenuated by fucoidan, which also protected against cytotoxicity evoked by buthionine-sulfoximine-mediated GSH depletion. Reduction in the glutathione/glutathione disulfide ratio induced by 6-OHDA was reversed by fucoidan, which also inhibited 6-OHDA-induced disruption of mitochondrial membrane potential. The results indicate that fucoidan may have protective action against 6-OHDAmediated neurotoxicity by modulating oxidative injury and apoptosis through GSH depletion.
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KEYWORD
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apoptosis, caspase-3/7, fucoidan, glutathione/glutathione disulfide ratio, 6-hydroxydopa
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